it causes inhibition away from its proposed mode of action. We conclude that the inhibition observed with this compound is due to non-specificity, i.e. In this study, we probed for non-specific activity of Pitstop 2 by examining its action in cells expressing clathrin heavy chain harbouring mutations in the N-terminal domain interaction sites. Despite this limitation, Pitstop 2 causes profound inhibition of clathrin-mediated endocytosis. “Pitstop” compounds have been developed that block only one of the four functional interaction sites on the N-terminal domain of clathrin heavy chain. One inhibition strategy is to prevent clathrin from contacting adaptor proteins so that clathrin-mediated endocytosis cannot occur. Click here.Small molecule inhibitors of clathrin-mediated endocytosis are highly desired for the dissection of membrane trafficking pathways in the lab and for potential use as anti-infectives in the clinic. In this case, you need to fix them in the source document.įor more information about settings and how to manage Acrobat Pro, visit the Adobe website. On the Profiles tab, you can try to fix the PDF by selecting the Analyze and Fix button, but this is not always possible (eg low-resolution images, missing fonts, etc.).By clicking the Create Report… button, you can save a detailed report on the compliance or errors (and their location) of the PDF. an orange triangle with an exclamation mark warns of non-critical errors,.the green check mark, of course, means that everything is fine with the PDF file,. After checking, you will get a list on the Results tab with the result:
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